The service quality of mobile health apps is a crucial factor affecting users’ satisfaction. For analyzing the influence of service quality of mobile health apps on users’ satisfaction in China, a hypothetical model with three dimensions and eight variables was established. Then this hypothetical model was combined with the model of mobile health service quality and hypotheses were proposed based on the actual situations according to the updated D&M information system success (UMDISS) theory. Questionnaires were issued to collect data and the SEM-PLS method was used to valid the hypothetical model and hypotheses. The reliability and security of the system quality of mobile health apps positively impacted the users’ satisfaction. The cooperation and personalization of service quality had a significant impact on the users’ willingness to use mobile health apps. Through multi-group analyses, it was found that the relationship between mobile health app system quality, users’ satisfaction, and the use of mobile health apps differed under different familiarities with mobile health apps. This study is expected to provide theoretical guidance for the marketing of mobile health apps.