This paper presents the design and implementation of a microwave based cavity based structure to determine the real-time glucose level based on the frequency shift. The glucose meter, which has an attenuation level greater than -30 dB using a single transmission pole, resulted in better selectivity and can be implemented for the measurement glucose concentration in an aqueous glucose solution. The noticeable variation in the resonance frequency as well as complex permittivity was achieved using the various glucose that validates the possibility of the devices as a glucose meter. The obtained parameters supports the development of resonance-based dielectric sensors using permittivity characterization with a resolution of 3.11 pF/mgdl-1 and a minimum detectable glucose level of 8.60 mgdl-1. This unique approach, which has long-term reliability for mediator-free glucose sensing, is the incremental step in the filed non-invasive glucose monitoring.